False Increases of Troponin I Attributable to Incomplete Separation of Serum

False increases of troponin I attributable to incomplete separation of serum.

To the Editor: In the process of establishing a troponin I assay in our laboratory, we found limited data on reference intervals for plasma. Because our laboratory relies heavily on plasma rather than serum to expedite analysis (1 ), we undertook a parallel study of serum and plasma troponin I concentrations with the Abbott AxSYM assay. To our surprise, we found several discordant results (Tabl...

False-negative immunoassay results for cardiac troponin I probably due to circulating troponin I autoantibodies.

False increase of cardiac troponin I with heterophilic antibodies.

Antibodies, Thomas F. Fitzmaurice, Charles Brown, Nader Rifai, Alan H.B. Wu, and Kiang-Teck J. Yeo ( Department of Pathology, Dartmouth-Hitchcock Medical Center and Dartmouth Medical School, 1 Medical Center Drive, Lebanon, NH 03756; 2 Department of Laboratory Medicine, Children’s Hospital & Harvard Medical School, Boston, MA 02115; and 3 Clinical Chemistry Laboratory, Hartford Hospital, Hartfo...

Serum troponin I as an indicator of myocarditis in lambs affected with foot and mouth disease

Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hooved livestock and wildlife results to relatively high mortality in young animals. Despite the numerous reports of FMD-related death in neonates, there is little data available on various aspects of FMD in lambs. This report describes myocarditis associated with FMD in five, one week to three months old lambs. The lam...

Degradation of cardiac troponin I in serum complicates comparisons of cardiac troponin I assays.

BACKGROUND Up to a 20-fold variation in serum cardiac troponin I (cTnI) concentration may be observed for a given patient sample with different analytical methods. Because more limited variation is seen for control materials and for purified cTnI, we explored the possibility that cTnI was present in altered forms in serum. METHODS We used four recombinantly engineered cTnI fragments to study ...